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Nucleic Acids Res. 1991 Aug 25;19(16):4377-85.

High-level ribosomal frameshifting directs the synthesis of IS150 gene products.

Author information

1
Institut für Biologie III, Universität Freiburg, FRG.

Abstract

IS150 contains two tandem, out-of-phase, overlapping genes, ins150A and ins150B, which are controlled by the same promoter. These genes encode proteins of 19 and 31 kD, respectively. A third protein of 49 kD is a transframe gene product consisting of domains encoded by both genes. Specific -1 ribosomal frameshifting is responsible for the synthesis of the large protein. Expression of ins150B also involves frameshifting. The IS150 frameshifting signals operate with a remarkably high efficiency, causing about one third of the ribosomes to switch frame. All of the signals required for this process are encoded in a 83-bp segment of the element. The heptanucleotide A AAA AAG and a potential stem-loop-forming sequence mark the frameshifting site. Similar sequence elements are found in -1 frameshifting regions of bacterial and retroviral genes. A mutation within the stem-loop sequence reduces the rate of frameshifting by about 80%. Artificial transposons carrying this mutation transpose at a normal frequency, but form cointegrates at a approximately 100-fold reduced rate.

PMID:
1653413
PMCID:
PMC328623
DOI:
10.1093/nar/19.16.4377
[Indexed for MEDLINE]
Free PMC Article

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