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Arch Biochem Biophys. 2006 Mar 15;447(2):97-106. Epub 2006 Feb 24.

Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes.

Author information

1
Institute of Biochemistry and Molecular Medicine, University of Bern, B├╝hlstrasse 28, 3012 Bern, Switzerland.

Abstract

In skin, vitamin E acts as the predominant lipophilic antioxidant with a protective function against irradiation and oxidative stress. In addition to that, vitamin E can also modulate signal transduction and gene expression. To study whether the four natural tocopherol analogues (alpha-, beta-, gamma-, delta-tocopherol) can influence transcriptional activity by modulating the activity of nuclear receptors, a human keratinocytes cell line (NCTC 2544) was transfected with plasmids containing the luciferase reporter gene under control by direct repeat elements (DR1-DR4), representing binding sites for four different classes of nuclear receptors. In this model, the tocopherols positively modulated only the reporter construct containing a consensus element for peroxisome proliferator-activated receptors (PPARs). The induction was strongest with gamma-tocopherol and was most likely the direct consequence of stimulation of PPARgamma protein expression in keratinocytes. Vitamin E treatment also led to increased expression of a known PPARgamma target gene involved in terminal keratinocytes differentiation, the transglutaminase-1.

PMID:
16530159
DOI:
10.1016/j.abb.2006.02.002
[Indexed for MEDLINE]

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