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Biochem Biophys Res Commun. 2006 Apr 28;343(1):85-9. Epub 2006 Feb 28.

Multiplexing RT-PCR for the detection of multiple miRNA species in small samples.

Author information

1
Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404, USA. laokq@appliedbiosystems.com

Abstract

MicroRNAs are short (approximately 22 nucleotides), non-coding RNAs that play critical roles in gene regulation and may be used as rapid precise diagnostic indicators of early stages of cancer. The small size of these RNAs makes detection of multiple microRNA species in very small samples problematic. Here we investigate the parameters associated with multiplexing RT-PCR to obtain relative abundance profiles of multiple microRNAs in small sample sizes down to the amount of RNA found in a single cell.

PMID:
16529715
DOI:
10.1016/j.bbrc.2006.02.106
[Indexed for MEDLINE]

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