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Science. 2006 Mar 10;311(5766):1464-7.

Structure and mechanism of the lantibiotic cyclase involved in nisin biosynthesis.

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1
Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.

Abstract

Nisin is a posttranslationally modified antimicrobial peptide that is widely used as a food preservative. It contains five cyclic thioethers of varying sizes that are installed by a single enzyme, NisC. Reported here are the in vitro reconstitution of the cyclization process and the x-ray crystal structure of the NisC enzyme. The structure reveals similarities in fold and substrate activation with mammalian farnesyl transferases, suggesting that human homologs of NisC posttranslationally modify a cysteine of a protein substrate.

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PMID:
16527981
DOI:
10.1126/science.1121422
[Indexed for MEDLINE]
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