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Tuberculosis (Edinb). 2006 May-Jul;86(3-4):310-3. Epub 2006 Mar 9.

Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library.

Author information

1
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Transposon mutagenesis produces random mycobacterial mutants at high frequency. Because they are random, however, it is difficult to isolate mutations in particular target genes. Here we describe the use of an arrayed library of Mycobacterium tuberculosis together with a PCR screening strategy to rapidly identify strains with defined insertion mutations. This method is useful for many genetic applications.

PMID:
16527544
DOI:
10.1016/j.tube.2006.01.015
[Indexed for MEDLINE]

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