Format

Send to

Choose Destination
See comment in PubMed Commons below
EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9.

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.

Author information

1
Department of Biological Sciences, Membrane Protein Crystallography Group, Imperial College London, London, UK.

Erratum in

  • EMBO J. 2006 May 3;25(9):2038.

Abstract

Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

PMID:
16525509
PMCID:
PMC1422171
DOI:
10.1038/sj.emboj.7601028
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center