Format

Send to

Choose Destination
FEBS Lett. 1991 Aug 5;287(1-2):211-4.

Site-directed mutagenesis of La protease. A catalytically active serine residue.

Author information

1
Shemyakin Institute of Biorganic Chemistry, USSR Academy of Sciences, Moscow.

Abstract

Comparative sequence analysis of Escherichia coli ATP-dependent La protease led to the suggestion that Ser679 is the catalytically active enzyme residue. Site-directed mutagenesis Ser679----Ala, investigation of the cells containing the mutant plasmid, and study of the partially purified mutant protein produced results in favour of this suggestion.

PMID:
1652461
DOI:
10.1016/0014-5793(91)80053-6
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center