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J Biomol Tech. 2005 Dec;16(4):316-7.

RNA isolation from yeast using silica matrices.

Author information

1
Department of Biochemistry, Faculty of Medicine and Dentistry, University of Western Ontario, London, Canada.

Abstract

RNA isolation from yeast is complicated by the need to initially break the cell wall. While this can be accomplished by glass bead disruption or enzyme treatment, these approaches result in DNA contamination and/or the need for incubation periods. We have developed a protocol for the isolation of RNA samples from yeast that minimizes degradation by RNases and incorporates two purification steps: acid phenol extraction and binding to a silica matrix. The procedure requires no precipitation steps, facilitating automation, and can be completed in less than 90 min. The RNA quality is ideal for microarray analysis.

PMID:
16522851
PMCID:
PMC2291748
[Indexed for MEDLINE]
Free PMC Article

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