Format

Send to

Choose Destination
J Proteome Res. 2006 Mar;5(3):695-700.

Effects of modified digestion schemes on the identification of proteins from complex mixtures.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA.

Abstract

In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and identified by microcapillary liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to a standard digestion protocol demonstrate large, reproducible improvements in protein identification, a result consistent with digestion being a limiting factor in the efficiency of protein identification.

PMID:
16512685
PMCID:
PMC2535816
DOI:
10.1021/pr050315j
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center