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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt 5):493-5. Epub 2005 Apr 22.

Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri.

Author information

1
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508-000, São Paulo, SP, Brazil.

Abstract

Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram-negative pathogens. Here, the cloning, expression, purification and crystallization of the 182-residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P2(1) and crystals diffracted to 1.9 A resolution at a synchrotron source. The unit-cell parameters are a = 39.75, b = 91.88, c = 48.03 A, beta = 108.37 degrees. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple-wavelength anomalous dispersion technique and an interpretable electron-density map was obtained.

PMID:
16511077
PMCID:
PMC1952311
DOI:
10.1107/S1744309105010985
[Indexed for MEDLINE]
Free PMC Article

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