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Biochem Int. 1991 Jan;23(1):83-92.

Superoxide anion production by lipoamide dehydrogenase redox-cycling: effect of enzyme modifiers.

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Centro de Investigaciones Bioenerg├ęticas, Facultad de Medicina, Buenos Aires, Argentina.


Redox-cycling of porcine heart lipoamide dehydrogenase in the presence of NADH and oxygen produced O2-. (NADH-oxidase activity) as demonstrated by (a) reduction of cytochrome c; (b) reduction of the Fe(III)-ADP complex; (c) lucigenin luminescence and (d) the inhibitory effect of superoxide dismutase. NAD+ and p-chloromercuribenzoate inhibited O2-. generation whereas arsenite enhanced it. Comparison of heart and yeast enzyme preparations revealed a close correlation between lipoamide reductase and NADH-oxidase activities. It is concluded that O2-. production is a molecular property of lipoamide dehydrogenase.

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