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J Biosci Bioeng. 2006 Jan;101(1):83-6.

Solution-stirring method improves crystal quality of human triosephosphate isomerase.

Author information

1
SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Abstract

We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 A resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 A. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity.

PMID:
16503298
DOI:
10.1263/jbb.101.83
[Indexed for MEDLINE]

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