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Diabetologia. 2006 Apr;49(4):784-91. Epub 2006 Feb 25.

Mitochondria are impaired in the adipocytes of type 2 diabetic mice.

Author information

1
Graduate School of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-gu, Seoul, 136-701, South Korea.

Abstract

AIMS/HYPOTHESIS:

The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes.

MATERIALS AND METHODS:

Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice.

RESULTS:

Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity.

CONCLUSIONS/INTERPRETATION:

Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes.

PMID:
16501941
DOI:
10.1007/s00125-006-0170-2
[Indexed for MEDLINE]

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