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J Microsc. 2006 Feb;221(Pt 2):145-51.

Laser autofocusing system for high-resolution cell biological imaging.

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1
Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.

Abstract

Automated acquisition of high resolution, light microscope images of cells is becoming a common requirement in modern proteomic and cellomic research. A prerequisite for such microscopy is fine focus tuning, commonly optimized by multiple exposures, followed by image sharpness analysis. We describe here an extremely fast and accurate laser autofocusing system with distinct advantages for large-scale cell-based screening.

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