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Yao Xue Xue Bao. 2005 Nov;40(11):1033-6.

[Effect of protein kinase C on human melanoma A375-S2 cell death induced by evodiamine].

[Article in Chinese]

Author information

1
China-Japan Research Institute of Medical and Pharmaceutical Sciences.

Abstract

AIM:

To study the role of PKC in evodiamine-induced A375-S2 cell death.

METHODS:

Ratio of apoptosis induced by evodiamine was determined by TUNEL assay. MTT assay was carried out to assess cytotoxic effect of evodiamine. The influence on expression of ERK, phospho-ERK and Bcl-2 was detected by Western blotting analysis.

RESULTS:

TUNEL assay indicated that apoptosis was the type of A375-S2 cell death induced by evodiamine treatment for 24 h. Both staurosporine (inhibitor of PKC) and PD98059 (inhibitor of ERK) cooperated with evodiamine to further induce A375-S2 cell death. Evodiamine inhibited PKC activity, down-regulated the expression of ERK, phospho-ERK and Bcl-2, and staurosporine was capable of augmenting these effects induced by evodiamine.

CONCLUSION:

PKC lies upstream and exhibits regulatory effect on ERK and Bcl-2 in evodiamine-induced cell death.

PMID:
16499090
[Indexed for MEDLINE]

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