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Yeast. 2006 Feb;23(3):173-83.

Basic methods for fission yeast.

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1
Program in Molecular and Computational Biology, University of Southern California, 1050 Child's Way MCB201, Los Angeles, CA 90089-2910, USA. forsburg@usc.edu

Abstract

The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast. Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology.

PMID:
16498704
PMCID:
PMC5074380
DOI:
10.1002/yea.1347
[Indexed for MEDLINE]
Free PMC Article
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