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Prostate. 2006 Jun 1;66(8):886-94.

GREB1 is a novel androgen-regulated gene required for prostate cancer growth.

Author information

1
Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA. jimmyrae@umich.edu

Abstract

BACKGROUND:

Gene regulated in breast cancer 1 (GREB1) is a novel estrogen-regulated gene shown to play a pivotal role in hormone-stimulated breast cancer growth. GREB1 is expressed in the prostate and its putative promoter contains potential androgen receptor (AR) response elements.

METHODS:

We investigated the effects of androgens on GREB1 expression and its role in androgen-dependent prostate cancer growth.

RESULTS:

Real-time PCR demonstrated high level GREB1 expression in benign prostatic hypertrophy (BPH), localized prostate cancer (L-PCa), and hormone refractory prostate cancer (HR-PCa). Androgen treatment of AR-positive prostate cancer cells induced dose-dependent GREB1 expression, which was blocked by anti-androgens. AR binding to the GREB1 promoter was confirmed by chromatin immunoprecipitation (ChIP) assays. Suppression of GREB1 by RNA interference blocked androgen-stimulated LNCaP cell proliferation.

CONCLUSIONS:

GREB1 is expressed in proliferating prostatic tissue and prostate cancer, is regulated by androgens, and suppression of GREB1 blocks androgen-induced growth suggesting GREB1 may be critically involved in prostate cancer proliferation.

PMID:
16496412
DOI:
10.1002/pros.20403
[Indexed for MEDLINE]

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