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Infect Immun. 2006 Mar;74(3):1949-53.

Routine markerless gene replacement in Bacillus anthracis.

Author information

1
Division of Bacterial Products, CBER/FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.

Abstract

An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.

PMID:
16495572
PMCID:
PMC1418658
DOI:
10.1128/IAI.74.3.1949-1953.2006
[Indexed for MEDLINE]
Free PMC Article
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