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J Clin Pathol. 2006 Apr;59(4):417-23. Epub 2006 Feb 17.

Liver infiltrating mononuclear cells in children with type 1 autoimmune hepatitis.

Author information

1
División inmunogenética, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Buenos Aires, Argentina.

Abstract

OBJECTIVE:

To investigate infiltrating cells in the liver of children with type 1 autoimmune hepatitis (AH-1).

METHODS:

liver biopsies from 24 untreated AH-1 patients (14 children, 10 adults), five patients with hepatitis C virus related chronic hepatitis (HCV), and 10 control liver specimens (CL) were processed for immunohistochemical cell characterisation.

RESULTS:

Two different cell distribution patterns were detected in the liver of patients with AH-1: (1) CD4(+) and CD20(+) cells were found in the central areas of the portal tracts (portal distribution); (2) CD8(+) cells were observed at the periphery of the portal space (periportal distribution). Some cell subsets, like CD56, CD57, Fas-L, and Bak, showed a non-defined distribution pattern. The presence of two well defined patterns of cell distribution was not observed in HCV and CL (CD4(+), CD20(+), and CD8(+) cells were uniformly distributed in the portal space). In AH-1 and CL, the NK markers CD56 and CD57 were found scattered throughout the liver parenchyma. However, in HCV biopsies, CD56(+) cells were also clearly increased in both the portal and the periportal areas. Biopsies of AH-1 and HCV patients showed a uniform distribution of Fas-L and Bak in the portal and periportal areas, with Bak staining also detected in the hepatic parenchyma.

CONCLUSIONS:

Despite clinical and genetic differences, there was a similar distribution of liver infiltrating mononuclear cells in children and adults with AH-1. These results raise the possibility of reclassifying cryptogenic chronic hepatitis by immunohistochemical analysis of infiltrating liver cells.

PMID:
16489183
PMCID:
PMC1860380
DOI:
10.1136/jcp.2005.028613
[Indexed for MEDLINE]
Free PMC Article
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