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Plant Physiol. 2006 Apr;140(4):1246-54. Epub 2006 Feb 17.

Chemical imaging of poplar wood cell walls by confocal Raman microscopy.

Author information

1
Max-Planck-Institute of Colloids and Interfaces, Department of Biomaterials, 14424 Potsdam, Germany. gierlinger@mpikg.mpg.de

Abstract

Confocal Raman microscopy was used to illustrate changes of molecular composition in secondary plant cell wall tissues of poplar (Populus nigra x Populus deltoids) wood. Two-dimensional spectral maps were acquired and chemical images calculated by integrating the intensity of characteristic spectral bands. This enabled direct visualization of the spatial variation of the lignin content without any chemical treatment or staining of the cell wall. A small (0.5 microm) lignified border toward the lumen was observed in the gelatinous layer of poplar tension wood. The variable orientation of the cellulose was also characterized, leading to visualization of the S1 layer with dimensions smaller than 0.5 mum. Scanning Raman microscopy was thus shown to be a powerful, nondestructive tool for imaging changes in molecular cell wall organization with high spatial resolution.

PMID:
16489138
PMCID:
PMC1435827
DOI:
10.1104/pp.105.066993
[Indexed for MEDLINE]
Free PMC Article
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