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FEBS Lett. 1991 Jul 8;285(1):139-44.

Expression of biologically active hormone-sensitive lipase in mammalian (COS) cells.

Author information

1
Department of Medical and Physiological Chemistry 4, University of Lund, Sweden.

Abstract

cDNAs encoding rat adipose tissue hormone-sensitive lipase were expressed in COS cells, under the control of the SV40 promoter to half the level in rat adipocytes, the richest native source of the enzyme. A cDNA lacking most of the long 5'-untranslated region of the full-length rat hormone-sensitive lipase cDNA was, with regard to the lipase activity, on the average 70% more efficiently expressed that the full-length cDNA. The recombinant protein was almost identical to hormone-sensitive lipase of rat adipose tissue with respect to specific activity, susceptibility to inhibitors, molecular size, phosphorylation and activation by cyclic AMP-dependent protein kinase. The described eukaryotic expression system will allow analysis of effects of amino acid substitutions introduced into the lipase molecule by site-directed mutagenesis.

PMID:
1648510
DOI:
10.1016/0014-5793(91)80744-n
[Indexed for MEDLINE]
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