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Nucleic Acids Res. 2006 Feb 9;34(3):e20.

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces.

Author information

1
Institute of Genetics, University of Nottingham Nottingham NG7 2UH, UK.

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phiC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

PMID:
16473843
PMCID:
PMC1363781
DOI:
10.1093/nar/gnj019
[Indexed for MEDLINE]
Free PMC Article

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