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Methods Enzymol. 2006;406:425-37.

Analysis of activated GAPs and GEFs in cell lysates.

Author information

1
Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA.

Abstract

An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals.

PMID:
16472675
DOI:
10.1016/S0076-6879(06)06031-9
[Indexed for MEDLINE]

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