Requirement of heparan sulfate for bFGF-mediated fibroblast growth and myoblast differentiation

Science. 1991 Jun 21;252(5013):1705-8. doi: 10.1126/science.1646484.

Abstract

Basic fibroblast growth factor (bFGF) binds to heparan sulfate proteoglycans at the cell surface and to receptors with tyrosine kinase activity. Prevention of binding between cell surface heparan sulfate and bFGF (i) substantially reduces binding of fibroblast growth factor to its cell-surface receptors, (ii) blocks the ability of bFGF to support the growth of Swiss 3T3 fibroblasts, and (iii) induces terminal differentiation of MM14 skeletal muscle cells, which is normally repressed by fibroblast growth factor. These results indicate that cell surface heparan sulfate is directly involved in bFGF cell signaling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Division
  • Cell Line
  • Chlorates / pharmacology
  • Fibroblast Growth Factor 2 / metabolism*
  • Fibroblasts / cytology*
  • Heparitin Sulfate / physiology*
  • In Vitro Techniques
  • Mice
  • Muscles / cytology*
  • Polysaccharide-Lyases / pharmacology
  • Protein Binding
  • Receptors, Cell Surface / metabolism
  • Structure-Activity Relationship

Substances

  • Chlorates
  • Receptors, Cell Surface
  • Fibroblast Growth Factor 2
  • Heparitin Sulfate
  • Polysaccharide-Lyases
  • heparitinsulfate lyase
  • sodium chlorate