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Anim Reprod Sci. 2007 Jan;97(1-2):74-83. Epub 2006 Feb 7.

Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa.

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Section of Animal Reproduction, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avd. de la Universidad s/n, 10071 Cáceres, Spain.


A recently developed triple staining (SNARF-1/YO-PRO-1/ethidium homodimer) was used to assess early changes in boar sperm membrane integrity (MI) with the results of cryopreservation procedures and to seek for correlations among MI-spermatozoa in pre-freeze semen and its freezeability. Ejaculates from five boars were evaluated in the fresh and frozen-thawed (FT) state, and its freezeability defined as % of membrane intactness, MI% (MI%=% of FT-spermatozoa with intact membranes x 100 divided by the % of pre-freeze spermatozoa with intact membranes) estimated. Significant differences were found among boars for freezeability (MI%) and motility post-thaw (%). Interestingly, significant correlations were found between the percentage of YO-PRO-1-positive spermatozoa and freezeability (R=0.440, p<0.01), indicating this new triple staining can be used to safely disclose among ejaculates prior to freezing.

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