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J Electron Microsc Tech. 1991 Mar;17(3):336-43.

Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study.

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Max-Planck-Institut für Biologie, Tübingen, Federal Republic of Germany.


In order to exploit the recently introduced 1 nm gold colloids in routine electron microscopic labeling experiments, an efficient enhancement step for a better visualization of this small marker is a prerequisite. Efficiency and reproducibility of enhancement as well as growth homogeneity of gold particles were evaluated for three different silver intensifying solutions: silver lactate/hydroquinone/gum arabic (Danscher, 1981), Ilford L4/Metol (Bienz et al., 1986), and the commercially available IntenSE M silver enhancement kit (Janssen Pharmaceutica). The best results were obtained by using the silver lactate/hydroquinone/gum arabic mixture. The quality of enhancement of the IntenSE M kit was considerably increased by the addition of the protective colloid gum arabic.

[Indexed for MEDLINE]

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