Format

Send to

Choose Destination
EMBO J. 1983;2(12):2253-8.

The nucleotide sequence of cowpea mosaic virus B RNA.

Author information

1
Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, UK.

Abstract

The complete sequence of the bottom component RNA (B RNA) of cowpea mosaic virus (CPMV) has been determined. Restriction enzyme fragments of double-stranded cDNA were cloned in M13 and the sequence of the inserts was determined by a combination of enzymatic and chemical sequencing techniques. Additional sequence information was obtained by primed synthesis on first strand cDNA. The complete sequence deduced is 5889 nucleotides long excluding the 3' poly(A), and contains an open reading frame sufficient to code for a polypeptide of mol. wt. 207 760. The coding region is flanked by a 5' leader sequence of 206 nucleotides and a 3' non-coding region of 82 residues which does not contain a polyadenylation signal.

PMID:
16453487
PMCID:
PMC555442

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center