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J Eur Acad Dermatol Venereol. 2006 Feb;20(2):149-52.

The alteration of sister chromatid exchange frequencies in Behçet's disease with and without HLA-B51.

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1
Ataturk University, Medical Faculty, Department of Medical Genetics, Erzurum, Turkey.

Abstract

BACKGROUND:

The analysis of sister chromatid exchange (SCE) is a cytogenetic technique used to show DNA damage as a result of an exchange of DNA fragments between sister chromatids. It is known that there is an increased SCE frequency in Behçet's disease (BD).

OBJECTIVE:

To investigate whether human leucocyte antigen (HLA)-B51-positive patients with Behçet's disease exhibit higher SCE frequencies than those without HLA-B51.

METHODS:

Lymphocytes from 75 patients (38 women, 37 men) and from 50 controls (28 women, 22 men) were cultured in darkness for 72 h in the presence of bromodeoxyuridine. Metaphase chromosomes were stained with a fluorescence plus Giemsa technique after a standard harvest procedure. For HLA-B51 typing, DNA was extracted from ethylenediaminetetraacetic acid blood samples and HLA-B5 allele genotyping was performed by the polymerase chain reaction (PCR)-sequence specific primer method.

RESULTS:

Thirty-nine of 75 patients with BD (52%) and 15 of 50 controls (30%) were found HLA-B51-positive. The SCE frequencies in HLA-B51-positive patients were higher than in HLA-B51-negative ones (P < 0.001), whereas no difference was detected in the control group.

CONCLUSION:

This study revealed that there was a significant association between elevated SCE frequencies and existence of HLA-B51 patients with BD.

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