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Mol Cell Biol. 2006 Feb;26(3):1038-50.

Low K+ promotes NF-kappaB/DNA binding in neuronal apoptosis induced by K+ loss.

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1
Laboratory of Neural Signal Transduction, Institute of Neuroscience, SIBS, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, People's Republic of China.

Abstract

Low intracellular K+ concentration ([K+]i) promotes apoptosis and blocking K+ loss prevents apoptosis, but the mechanism of action of low [K+]i remains unclear. Here, we show that low [K+]i increases NF-kappaB transcriptional activity by enhancing its binding to the promoter of target genes without affecting its activation and nuclear translocation in cortical neurons deprived of serum. Low K+ concentration promotes NF-kappaB/DNA binding through direct effects on the interaction of NF-kappaB dimers with DNA. Up-regulation of proapoptotic protein Bcl-XS and neuronal apoptosis induced by serum deprivation are blocked by inhibition and/or down-regulation of NF-kappaB and by prevention of K+ loss. Thus, a direct action of K+ on NF-kappaB/DNA binding regulates gene transcription related to neuronal apoptosis.

PMID:
16428456
PMCID:
PMC1347023
DOI:
10.1128/MCB.26.3.1038-1050.2006
[Indexed for MEDLINE]
Free PMC Article
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