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Neoplasma. 2006;53(1):1-8.

Suppression of glucosylceramide synthase by RNA interference reverses multidrug resistance in human breast cancer cells.

Author information

1
Department of Pathology, School of Medicine, Shandong University, 250012 Jinan, China. yanlinsunny@yahoo.com.cn

Abstract

Glucosylceramide synthase (GCS), the enzyme that converts ceramide to glucosylceramide, induce multidrug resistance (MDR) in cancer cells. Recently, RNA interference (RNAi) is a powerful strategy for gene therapy by introducing double-stranded RNA and leading to the sequence-specific destruction. We have designed two different short hairpin RNAs (shRNAs) targeting GCS and introduced them into adriamycin- resistant human breast cancer cells (MCF-7/AdrR cells) to inhibit GCS expression. The results demonstrated that the shRNAs targeting GCS decreased GCS mRNA, abolished GCS protein levels and restored the sensitivity of MCF-7/AdrR cells to several antineoplastic drugs. This study revealed that this approach can reverse MDR effectively and it may be applicable to cancer patients as a specific means to restore the sensitivity to chemotherapy.

PMID:
16416005
[Indexed for MEDLINE]

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