Format

Send to

Choose Destination
Methods Enzymol. 2005;404:95-107.

Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size.

Author information

1
Institut de Pharmacologie, Moleculaire et Cellulaire, CNRS, Valbonne, France.

Abstract

The assembly-disassembly cycle of COPI and COPII coats is controlled by the GTPase cycle of the small G proteins Arf1 and Sar. We describe here two spectroscopic assays that enable real-time studies of some elementary steps of coat assembly and disassembly on artificial liposomes of defined composition and curvature. A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented.

PMID:
16413261
DOI:
10.1016/S0076-6879(05)04010-3
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center