Abstract
The pectic polysaccharide (angelan) of Angelica gigas Nakai is an immunostimulator that activates the immune functions of B cells and macrophages. Here we investigated the effect of angelan on tumor growth and metastasis. Angelan was found to significantly prolong the survival rate of B16F10-implanted mice and to reduce the frequency of pulmonary metastasis of B16F10 melanoma. Moreover, the combined treatment of angelan and doxorubicin (a cytotoxic anticancer agent) more effectively inhibited tumor growth and metastasis than either compound alone. In the present study, we found that angelan directly inhibited cancer cell adhesion and invasion through the extracellular matrix, in addition to activating the immune functions of B cells and macrophages. These results suggest that angelan can inhibit tumor growth and metastasis by stimulating host immunity and directly inhibiting cancer cell adhesion.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Angelica / chemistry*
-
Animals
-
B-Lymphocytes / drug effects
-
B-Lymphocytes / immunology
-
Cell Adhesion / drug effects
-
Cell Line
-
Cell Line, Tumor
-
Cell Movement / drug effects
-
Cell Proliferation / drug effects*
-
Cell Survival / drug effects
-
Dose-Response Relationship, Drug
-
Doxorubicin / administration & dosage
-
Doxorubicin / pharmacology
-
Female
-
Injections, Intraperitoneal
-
Macrophages / drug effects
-
Macrophages / immunology
-
Melanoma, Experimental / mortality
-
Melanoma, Experimental / pathology
-
Melanoma, Experimental / prevention & control*
-
Mice
-
Mice, Inbred C57BL
-
Neoplasm Metastasis / immunology
-
Neoplasm Metastasis / pathology
-
Neoplasm Metastasis / prevention & control*
-
Plant Preparations / administration & dosage
-
Plant Preparations / pharmacology
-
Polysaccharides / administration & dosage
-
Polysaccharides / pharmacology*
-
Survival Rate
-
T-Lymphocytes / drug effects
-
T-Lymphocytes / immunology
Substances
-
Plant Preparations
-
Polysaccharides
-
angelan
-
Doxorubicin