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FEBS Lett. 2006 Jan 23;580(2):711-5. Epub 2005 Dec 28.

Transcription factor cycling on the insulin promoter.

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School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.


Using MIN6 beta-cells and chromatin immunoprecipitation (ChIP) assays, the chronological sequence of binding of MafA, E47/beta2 and PDX-1 to the insulin promoter in living beta-cells were investigated. All four factors were shown to bind to the mouse insulin 2 promoter in a cyclical manner with a periodicity of approximately 10-15 min. The cyclical binding of MafA, E47 and beta2 was largely unaffected by the glucose or insulin concentration in the media. However, the binding and cycling of PDX-1 was markedly abolished in low glucose (1 mM), and this was reversed in the presence of low concentrations of insulin.

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