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Magn Reson Imaging. 2006 Jan;24(1):27-31. Epub 2005 Dec 20.

Compatibility of Gd-DTPA perfusion and histologic studies of the brain.

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1
Nuclear Magnetic Resonance Unit, National Institute on Aging, Intramural Research Program, Baltimore, MD 21224, USA. spencer@helix.nih.gov

Abstract

Histology, including immunohistochemistry, and magnetic resonance imaging microscopy (microMRI) are complementary techniques for the analysis of brain structure. Therefore, microMRI analysis, often of formalin-fixed tissue, precedes histologic evaluation of the same experimental animal in many studies. However, the application of gadopentetate dimeglumine (Gd-DTPA), while of value for MRI studies, has an unknown effect on subsequent histology. We demonstrate here that for the mouse brain, histology with Nissl staining and immunostaining for microtubule-associated protein 2, using standard techniques for tissue preparation, are unaffected by prior perfusion of the tissue with Gd-DTPA. This conclusion was based on qualitative morphologic comparisons of stained sections, as well as quantification of mean immunofluorescence pixel intensities from Gd-treated (mean+/-S.D.=131.2+/-28.4; n=3) as compared to nontreated specimens (116.2+/-34.7; n=3, P=.7). Therefore, Gd-DTPA may be applied as a microMRI contrast agent in formalin-fixed brain tissue prior to histologic studies.

PMID:
16410175
DOI:
10.1016/j.mri.2005.10.017
[Indexed for MEDLINE]
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