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Nat Chem Biol. 2005 Oct;1(5):263-4. Epub 2005 Sep 18.

High-throughput assay for small molecules that modulate zebrafish embryonic heart rate.

Author information

1
Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA. gburns@cvrc.mgh.harvard.edu

Abstract

To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.

PMID:
16408054
DOI:
10.1038/nchembio732
[Indexed for MEDLINE]

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