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J Clin Virol. 2006 Apr;35(4):463-6. Epub 2006 Jan 6.

Association of respiratory picornaviruses with acute bronchiolitis in French infants.

Author information

1
Laboratoire de Virologie, Service de Microbiologie, Hôpital Robert Debré, Avenue du Général Koenig, 51092 REIMS Cedex, France.

Abstract

BACKGROUND:

Human rhinoviruses and enteroviruses (Picornaviridae) are suspected to be major viral etiological causes of bronchiolitis in infants.

OBJECTIVES:

In the present study, we assessed the potential role of the respiratory picornaviruses as causative agents of bronchiolitis in French infants.

STUDY DESIGN:

From September 2001 to June 2002, we prospectively selected 192 infants < or =36 months of age and hospitalized for acute bronchiolitis. The detection of common respiratory viruses (respiratory syncytial virus, influenza virus A and B, parainfluenza virus 1, 2, 3 and adenovirus) was performed using classical immunofluorescence antigen and cell-culture detection assays on nasopharyngeal aspirates whereas the detection of human metapneumovirus (HMPV) was performed by a real-time RT-PCR assay. The presence of rhinovirus and/or enterovirus was assessed in respiratory samples by a picornavirus RT-PCR detection assay followed by a differential Southern blotting procedure.

RESULTS:

A potential causative virus was detected in 72.5% of the 192 study infants. RSV (30%), rhinovirus (21%), enterovirus (9%), influenza virus A (6%) and human metapneumovirus (4%) were the most frequent causative agents detected. Rhinoviruses or enteroviruses were detected as the only evidence of respiratory viral tract infection in 57 (30%) of 192 infants, whereas rhinovirus or enterovirus occurred in mixed viral infection detected in 25 (13%) of 192 study cases (30% versus 13%, p<10(-3)).

CONCLUSIONS:

Our data suggest that respiratory picornaviruses are one of the leading etiological causes of bronchiolitis in French infants. These findings highlight the need to implement a rapid picornavirus RT-PCR detection assay for the clinical diagnosis of respiratory infections in pediatric patients with bronchiolitis.

PMID:
16406692
DOI:
10.1016/j.jcv.2005.11.009
[Indexed for MEDLINE]

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