Format

Send to

Choose Destination
See comment in PubMed Commons below
Mass Spectrom Rev. 2006 Mar-Apr;25(2):327-44.

Peptidomics: identification and quantification of endogenous peptides in neuroendocrine tissues.

Author information

1
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. fricker@aecom.yu.edu

Abstract

Neuropeptides perform a large variety of functions as intercellular signaling molecules. While most proteomic studies involve digestion of the proteins with trypsin or other proteases, peptidomics studies usually analyze the native peptide forms. Neuropeptides can be studied by using mass spectrometry for identification and quantitation. In many cases, mass spectrometry provides an understanding of the precise molecular form of the native peptide, including post-translational cleavages and other modifications. Quantitative peptidomics studies generally use differential isotopic tags to label two sets of extracted peptides, as done with proteomic studies, except that the Cys-based reagents typically used for quantitation of proteins are not suitable because most peptides lack Cys residues. Instead, a number of amine-specific labels have been created and some of these are useful for peptide quantitation by mass spectrometry. In this review, peptidomics techniques are discussed along with the major findings of many recent studies and future directions for the field.

PMID:
16404746
DOI:
10.1002/mas.20079
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center