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Nucleic Acids Res. 2006 Jan 5;34(1):e2.

Fast chromatin immunoprecipitation assay.

Author information

1
Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA.

Abstract

Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.

PMID:
16397291
PMCID:
PMC1325209
DOI:
10.1093/nar/gnj004
[Indexed for MEDLINE]
Free PMC Article
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