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Chem Commun (Camb). 2006 Jan 21;(3):260-2. Epub 2005 Nov 28.

Intein-mediated in vitro synthesis of lipidated Ras proteins.

Author information

1
Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227, Dortmund, Germany.

Abstract

Fully functional lipid-modified Ras proteins suitable for the study of Ras-membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.

PMID:
16391727
DOI:
10.1039/b511736d
[Indexed for MEDLINE]

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