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Nucleic Acids Res. 2006 Jan 1;34(Database issue):D207-12.

The REFOLD database: a tool for the optimization of protein expression and refolding.

Author information

1
Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia.

Abstract

A large proportion of proteins expressed in Escherichia coli form inclusion bodies and thus require renaturation to attain a functional conformation for analysis. In this process, identifying and optimizing the refolding conditions and methodology is often rate limiting. In order to address this problem, we have developed REFOLD, a web-accessible relational database containing the published methods employed in the refolding of recombinant proteins. Currently, REFOLD contains >300 entries, which are heavily annotated such that the database can be searched via multiple parameters. We anticipate that REFOLD will continue to grow and eventually become a powerful tool for the optimization of protein renaturation. REFOLD is freely available at http://refold.med.monash.edu.au.

PMID:
16381847
PMCID:
PMC1347443
DOI:
10.1093/nar/gkj080
[Indexed for MEDLINE]
Free PMC Article

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