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Mycol Res. 2006 Feb;110(Pt 2):188-95. Epub 2005 Dec 27.

Isolation and characterisation of Beauveria bassiana isolates from phylloplanes of hedgerow vegetation.

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  • 1Department of Ecology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.


A leaf imprinting technique combined with a selective medium was used to document the natural occurrence of Beauveria bassiana on phylloplanes of typical hedgerow plants (grasses, stinging nettle and hawthorn) in May, July and September in a hedgerow in Denmark. The density of B. bassiana (as measured by numbers of colony forming units) was greatest in September and on lower nettle leaves. B. bassiana was isolated from phylloplanes in a different hedgerow the following year and a similar picture of occurrence was found. Genetic diversity of selected in vitro isolates were characterised by Universally Primed (UP) PCR, and 13 distinguishable banding patterns were found at the two localities. Of these, four were shared between the field sites and all plant species harboured isolates of B. bassiana with at least two different banding patterns. The isolation method described represents a valuable tool for studying naturally occurring B. bassiana and for rapid isolation of indigenous strains of the fungus for future development of biocontrol agents. The significance of the findings for the life-cycle of B. bassiana is discussed.

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