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Nat Methods. 2006 Jan;3(1):27-9.

In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.

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1
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.

Abstract

MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.

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PMID:
16369549
DOI:
10.1038/nmeth843
[Indexed for MEDLINE]

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