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Retrovirology. 2005 Dec 20;2:79.

Involvement of claudin-7 in HIV infection of CD4(-) cells.

Author information

1
Jonsson Comprehensive Cancer Center, UCLA School of Dentistry, Dental Institute, Los Angeles, CA 90095, USA. zyzheng29@hotmail.com

Abstract

BACKGROUND:

Human immunodeficiency virus (HIV) infection of CD4(-) cells has been demonstrated, and this may be an important mechanism for HIV transmission.

RESULTS:

We demonstrated that a membrane protein, claudin-7 (CLDN-7), is involved in HIV infection of CD4(-) cells. A significant increase in HIV susceptibility (2- to 100-fold) was demonstrated when CLDN-7 was transfected into a CD4(-) cell line, 293T. In addition, antibodies against CLDN-7 significantly decreased HIV infection of CD4(-) cells. Furthermore, HIV virions expressing CLDN-7 on their envelopes had a much higher infectivity for 293T CD4(-) cells than the parental HIV with no CLDN-7. RT-PCR results demonstrated that CLDN-7 is expressed in both macrophages and stimulated peripheral blood leukocytes, suggesting that most HIV virions generated in infected individuals have CLDN-7 on their envelopes. We also found that CLDN-7 is highly expressed in urogenital and gastrointestinal tissues.

CONCLUSION:

Together these results suggest that CLDN-7 may play an important role in HIV infection of CD4(-) cells.

PMID:
16368003
PMCID:
PMC1352390
DOI:
10.1186/1742-4690-2-79
[Indexed for MEDLINE]
Free PMC Article

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