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Microbiol Immunol. 2005;49(12):1027-33.

Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster.

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Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.


Thirty-one epidemiologically unrelated Legionella pneumophila serogroup 1 isolates (10 from cooling towers, 10 from public spas and/or hot spring baths, and 11 from patients) were analyzed by pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) using 6 loci, flaA, pilE, asd, mip, mompS, and proA. The results of PFGE and SBT analysis indicated that all 10 isolates from cooling towers clustered into a unique type, which was distinct from strains of other environmental sources.

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