Abstract
Respiratory isolates of Klebsiella pneumoniae in Korea during 2002-2003 were studied to determine the prevalence and types of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamases (PABLs). ESBL-production was tested by double-disk synergy, and genotypes of beta-lactamases were determined by PCR and sequencing. ESBLs were detected in 28.4% of 373 isolates, and the most prevalent types were SHV-12 (63 isolates) and CTX-M-14 (9 isolates). Forty of 75 ESBL-producers (53.5%) also had PABLs: 21 isolates with CMY-2-like, 17 with DHA-1-like. Pulsed-field gel electrophoresis showed 19 types and 25 of 74 isolates had an identical pattern, indicating nosocomial spread. Dissemination of ESBL- and PABL-producing K. pneumoniae strains in Korea is a particular concern, as it limits the choice of antimicrobial agents for treatment of infections.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / therapeutic use
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / classification
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Bacterial Proteins / genetics
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Base Sequence
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Cross Infection / microbiology
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial
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Electrophoresis, Gel, Pulsed-Field
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Genes, Bacterial
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Humans
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Klebsiella Infections / drug therapy
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Klebsiella Infections / microbiology*
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Klebsiella pneumoniae / classification
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics
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Klebsiella pneumoniae / isolation & purification*
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Korea
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Respiratory Tract Infections / drug therapy
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Respiratory Tract Infections / microbiology*
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beta-Lactamases / biosynthesis
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beta-Lactamases / classification*
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beta-Lactamases / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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beta-lactamase CMY-2
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beta-lactamase CTX-M-14
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AmpC beta-lactamases
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beta-Lactamases