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Tuberculosis (Edinb). 2006 Nov;86(6):438-44. Epub 2005 Dec 15.

A derivative of Mycobacterium smegmatis mc(2)155 that lacks the duplicated chromosomal region.

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1
MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST-NRF, School of Pathology of the University of the Witwatersrand, Johannesburg, South Africa. digby.warner@nhls.ac.za <digby.warner@nhls.ac.za>

Abstract

The genome of Mycobacterium smegmatis mc(2)155 contains a 56kb duplicated region. We isolated a mutant of mc(2)155 lacking this duplication (DeltaDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of DeltaDRKIN for the study of genes contained within the duplicated region.

PMID:
16359921
DOI:
10.1016/j.tube.2005.10.001
[Indexed for MEDLINE]
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