Soluble starch was hydrolyzed to glucose by conidia of Aspergillus wentii NRRL 2001. Peak yields of glucose were achieved in 3 days. A glucoamylase-like enzyme was assumed to be responsible since maltose was not detected during the conversion. Spore age, storage conditions, and temperature affected the level of glucose accumulated. Iodoacetate inhibited catabolism of the glucose formed and this inhibition increased product yield. Spores of other fungi also hydrolyzed starch but none accumulated glucose naturally as did A. wentii spores.