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Appl Environ Microbiol. 1989 Jul;55(7):1818-22.

Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA.

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1
Department of Microbiology, Montana State University, Bozeman, Montana 59717.

Abstract

Cloning of cDNA obtained from 16S rRNA (16S rcDNA) selectively retrieves species-specific sequence information useful for analyzing the composition and structure of natural microbial communities. With this technique we obtained recombinant 16S rcDNA libraries from Escherichia coli and from a model hot-spring cyanobacterial-mat community. The recombinant plasmids contained exclusively 16S rRNA-derived inserts. This selective approach is independent of biasing culture techniques and eliminates the laborious screening required to locate 16S rRNA gene-bearing recombinants in genomic DNA libraries obtained from natural communities.

PMID:
16347975
PMCID:
PMC202956
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