Format

Send to

Choose Destination
Appl Environ Microbiol. 1986 Jun;51(6):1170-3.

Characterization of Leucine Auxotrophs of the White Rot Basidiomycete Phanerochaete chrysosporium.

Author information

1
Department of Chemical, Biological, and Environmental Sciences, Oregon Graduate Center, Beaverton, Oregon 97006-1999.

Abstract

Six leucine auxotrophic strains of the white rot basidiomycete Phanerochaete chrysosporium were characterized genetically and biochemically. Complementation studies involving the use of heterokaryons identified three leucine complementation groups. Since all of the leucine auxotrophs grew on minimal medium supplemented with alpha-ketoisocaproate as well as with leucine, the transaminase catalyzing the last step in the leucine pathway was apparently normal in all strains. Therefore, the wild-type, auxotrophic, and several heterokaryotic strains were assayed for the activities of the other enzymes specific to leucine biosynthesis. Leu2 and Leu4 strains (complementation group I) lacked only alpha-isopropylmalate synthase activity; Leu3 and Leu6 strains (group III) lacked isopropylmalate isomerase activity; and Leu1 and Leu5 strains (group II) lacked beta-isopropylmalate dehydrogenase. Heterokaryons formed from leucine auxotrophs of different complementation groups had levels of activity for all three enzymes similar to those found in the wild-type strain.

PMID:
16347073
PMCID:
PMC239040

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center