Microtubule-binding properties of dynactin p150 expedient for dynein motility

Biochem Biophys Res Commun. 2006 Feb 3;340(1):23-8. doi: 10.1016/j.bbrc.2005.11.145. Epub 2005 Dec 6.

Abstract

Dynactin is a hetero-oligomeric protein complex that has an important role in dynein-based intracellular transport. The expressed N-terminal fragments of dynactin p150 bound to microtubules in the ratio of one to one tubulin dimer, independent from the binding of dynein stalk head. Single molecule observation revealed that these fragments moved around on microtubules by Brownian motion. When the dynein-dynactin complex moves on microtubules, p150 can support dynein to maintain contact with microtubules and does not interfere with the motility of dynein, and thus, the dynein-dynactin complex can efficiently achieve long-distance carriage of the cargo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Dynactin Complex
  • Dyneins / chemistry*
  • Microtubule-Associated Proteins / chemistry*
  • Microtubules / chemistry*
  • Molecular Motor Proteins / chemistry*
  • Motion
  • Protein Binding
  • Protein Conformation
  • Structure-Activity Relationship

Substances

  • Dynactin Complex
  • Microtubule-Associated Proteins
  • Molecular Motor Proteins
  • Dyneins